International Journal of Innovation in Pharma Biosciences and Research Technology

Chief Editor Mrs. B.S. Jayachitra


Structural Identification and Antioxidant Properties of methanolic extract of brown algae Lobophora variegata (J.V. Lamouroux) Womersley ex E.C.Oliveir
Thennarasan Sathyaseelan1, Subbiah Murugesan1* and Vajiravelu Sivamurugan2*
The antioxidant ability of Lobophora variegate have been evaluated through 2, 2-diphenyl-1-picrylhydrazyl (DPPH), 2, 2- azino-bis 3- ethylbenzothiazoline-6 sulphuric acid (ABTS), Superoxide hydroxyl and Nitric oxide scavenging radicals. It has been observed that the methanolic extract of L. variegata showed a concentration dependent (between 100 and 900 g/mL). The IC50 values of ABTS scavenging of free-radical was 96 g/mL, DPPH 62 g/mL, Superoxide 74 g/mL, and Nitric oxide 84 g/mL and Hydroxyl radical was 74 g/mL. Among the five radicals, the ABTS showed the highest activity as compared to the other free-radicals, which indicating the dose dependency of the algal extract. Although the concentration of methanolic extract of L.variegata were much closer to the standard drugs (BHT and L-ascorbic acid) which used for the antioxidant studies as tested in the above five assays. The significant free radical scavenging activity of L.variegata indicated that it could be a potential source of natural antioxidant. The chemical structure of the active component (Compound 1) isolated from the methanolic extract has been identified using 1H NMR, 13C NMR, GC-MS and UV-Vis spectroscopy.
Keywords: Anti-oxidant, Free-radical, Lobophora variegata, Nutraceutical.
Corresponding author email id Full text Published Date : 12-Jun-2015