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International Journal of Innovation in Pharma Biosciences and Research Technology
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Chief Editor Mrs. B.S. Jayachitra

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Structural Identification and Antioxidant Properties of methanolic extract of brown algae Lobophora variegata (J.V. Lamouroux) Womersley ex E.C.Oliveir
Thennarasan Sathyaseelan1, Subbiah Murugesan1* and Vajiravelu Sivamurugan2*
Abstract:
The antioxidant ability of Lobophora variegate have been evaluated through 2, 2-diphenyl-1-picrylhydrazyl (DPPH), 2, 2- azino-bis 3- ethylbenzothiazoline-6 sulphuric acid (ABTS), Superoxide hydroxyl and Nitric oxide scavenging radicals. It has been observed that the methanolic extract of L. variegata showed a concentration dependent (between 100 and 900 g/mL). The IC50 values of ABTS scavenging of free-radical was 96 g/mL, DPPH 62 g/mL, Superoxide 74 g/mL, and Nitric oxide 84 g/mL and Hydroxyl radical was 74 g/mL. Among the five radicals, the ABTS showed the highest activity as compared to the other free-radicals, which indicating the dose dependency of the algal extract. Although the concentration of methanolic extract of L.variegata were much closer to the standard drugs (BHT and L-ascorbic acid) which used for the antioxidant studies as tested in the above five assays. The significant free radical scavenging activity of L.variegata indicated that it could be a potential source of natural antioxidant. The chemical structure of the active component (Compound 1) isolated from the methanolic extract has been identified using 1H NMR, 13C NMR, GC-MS and UV-Vis spectroscopy.
Keywords: Anti-oxidant, Free-radical, Lobophora variegata, Nutraceutical.
Corresponding author email id Full text Published Date : 12-Jun-2015